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Thiogalactoside

WebJun 1, 2005 · 1.. IntroductionThe genes of the classical lac operon of E. coli (lacZ, lacY, and lacA) encode β-galactosidase, lactose permease and thiogalactoside acetyltransferase [1].Although the catalytic activity of the LacA gene product was initially inferred from the study of thiogalactoside substrates, the subsequent determination of its broader … WebIPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% (by HPLC), white powder for genomic. Catalog Number: (IC11IPTG0001) Supplier: MP Biomedicals. Description: IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog tha... View Product Page.

蓝白斑筛选的原理及方法_百度文库

WebIsopropyl-β- d -thiogalactopyranoside (IPTG) is a kind of nonmetabolizable analog of lactose widely used for heterogeneous gene expression in Escherichia coli system. Although IPTG functions as an efficient inducer of the lac operator, its high cost and potential toxicity to … WebIPTG,英文全名Isopropyl-beta-D-thiogalactoside,中文全名异丙基-β-D-硫代半乳糖苷,CAS NO:367-93-1,诱导细菌lac或tac操纵子调控的基因转录,尤其是水解酶β-半乳糖苷酶(β-gal,beta-gal)。 一旦表达,β-gal水解β-半乳糖苷和乳糖为单糖。 jnrealtyinc.com https://rendez-vu.net

IPTG (Isopropyl Beta-D-Thiogalactoside) Inducer For Beta

WebIsopropyl-β-D-thiogalactoside. mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001) View Price and Availability. Sigma-Aldrich. A6426. Antifoam 204. mixture of organic polyether dispersions. View Price and Availability. Sigma-Aldrich. K1637. Kanamycin sulfate from Streptomyces kanamyceticus. WebIPTG. IPTG即Isopropyl β-D-1-thiogalactopyranoside,也称Isopropyl β-D-thiogalactoside,中文名为异丙基-β-D-硫代半乳糖苷。. 分子式为C9 H18 O5 S,分子量为238.30,CAS Number 367-93-1,Ultra Pure,dioxane free,纯度>99%。. 进口分装。. 本产品为接近白色的粉末。. 常用分子生物学试剂,常用 ... WebMeasurements have recorded the molar ratio of $\beta$-galactosidase : thiogalactoside transacetylase (the protein products of lacZ and lacA) upon induction of the operon at 3:1 to 5:1; i.e. when the lac operon is induced 3 to 5 molecules of $\beta$-gal are produced for every one of the transacetylase. jnr class 205

2-Phenylethyl 1-Thio-beta-D-thiogalactoside - PubChem

Category:Methyl β-D-thiogalactoside, 98%, Thermo Scientific™

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Thiogalactoside

A practical and scalable synthesis of KRN7000 using glycosyl …

Web当DNA片段插入至pUC系列载体(或其他带有lacZ、Amp基因载体),然后转化至lacZ缺失细胞中后,涂布上述的IPTG、X-gal、Amp平板培养基,可根据长出菌体的蓝白色,而方便地挑选出基因重组体(白色为具有DNA插入片段的基因重组体)。. 蓝白斑筛选的原理及方法. 载体 … WebThe invention relates to the field of sugar compounds, and concretely relates to an isopropyl-beta-D-thiogalactoside preparation method. The method comprises the following steps: adding acetic anhydride and a catalyst at room temperature, adding galactose, adding isopropyl mercaptan after the above reaction, and post-processing to obtain isopropyl …

Thiogalactoside

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Webthiogalactoside (plural thiogalactosides) (organic chemistry) A galactoside in which the … WebSynonym. methyl-beta-d-thiogalactoside, methyl-1-thio-beta-d-galactopyranoside, methyl 1 …

WebMar 24, 2015 · IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. IPTG, Dioxane-Free is widely used with X-Gal (sc-280488) plates to screen for blue and white colonies. WebNational Center for Biotechnology Information

WebNov 30, 2015 · Expression fusionprotein -fusionprotein, 10 ml overnightculture coliBL21(DE3) containing pET-5a-AP1 inocu-late Luria–Bertanibroth supplemented ampicillin.Aculture containing constructincubated under sameconditions control.After incubation 0.4mM IPTG (isopropyl β-D-thiogalactoside) Cellswere harvested …

WebThe invention relates to the field of saccharide compounds, particularly a method for preparing isopropyl-beta-D-thiogalactoside. The method comprises the following steps: adding acetic anhydride and a catalyst at room temperature, and adding galactose; after the reaction finishes, adding isopropyl mercaptan; after the reaction finishes, carrying out …

WebView our Thioglycosides products at Fisher Scientific. Please sign in to view account … jnr choi \\u0026 sam tompkins - to the moonWebThiodigalactoside (TDG)is a potent inhibitor of galectin-1 (GAL1) that suppress tumor … jnr city centreWebThiodigalactoside C12H22O10S CID 119138 - structure, chemical names, physical and … institute of english kerala universityWebThe uptake of methyl-β-D-thiogalactoside (TMG), lactose, and glucose is maintained by … jnr class dd51WebView our Thioglycosides products at Fisher Scientific. Please sign in to view account pricing and product availability. Sign In jnr class c55WebThiogalactoside transacetylase, one of the products of the lac operon in Escherichia coli, … institute of event planningWebMethyl-β-D-thiogalactoside has been used in a study to analyze inducers of the E.coli lac repressor system. It has also been used in a study that investigated the utilization of lactose by Streptococcus faecalis. Packaging. 100, 250, 500 mg. 1, 5 g. Bottomless glass bottle. Contents are inside inserted fused cone. institute of estate agents singapore