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Plvx-shrna2

WebFeb 1, 2024 · The targets of LACTB shRNA sequences were 5′-CCATGGTTGCTCTTGCCAA-3′ and 5′-GCCATAGTAGAGAGAGCTT-3’. Oligos containing the scrambled or non-targeted … WebDec 3, 2024 · The pLVX-shRNA2 and pLVX-IRES-ZsGreen1 plasmids (Clontech, USA) were selected as the lentiviral vectors for PC4 knockdown and overexpression, respectively. The target sequences for PC4 knockdown were PC4 sh1 GAAGGAACAGATTTCTGACAT; PC4 sh2 GACAGGTGAGACTTCGAGA; and PC4 sh3 GCAAAGTGCTAATTGATAT.

CCR6 Is a Prognostic Marker for Overall Survival in Patients

WebMar 1, 2024 · The pLVX‐shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX‐shRNA2 and psi‐LVRU6GP plasmids were associated with efficient RNA interference in... WebJan 21, 2024 · The correct vector plasmid packaging virus was identified. The lentiviral shRNA-expressing plasmids pLVX-shRNA2-puro, Amp + (Biowit Technology, Shenzhen, China) were transfected with the packaging plasmids into 293T cells for lentivirus generation . The viral supernatants were harvested, and 293T cells were used to … quelle heure est-il a washington https://rendez-vu.net

TOE1敲低和过表达稳转细胞系的构建及其在胃癌细胞中的定位分析

WebMaterials and methods: pLVX-shRNA2 lentiviral vector system was established, packaged and transfected into H-ILCSCs, HCCLM3 and HL-7702 cells. Cell counting kit-8 (CCK-8) assay was performed to observe cell viabilities of cells. Transwell assay was conducted to evaluate the invasion potential of H-ILCSCs, HCCLM3 and HL-7702 cells. WebJun 7, 2014 · The TβRII shRNA or negative control sequence was inserted into plvx-shRNA2 vectors (Clontech) using Bam H1 and Eco R1 restriction sites, packed according to the manufacturer’s instructions (Clontech, USA). To make lentiviral particles, ViraPower Lentiviral Expression System (Invitrogen) was used. WebpLVX-shRNA1 Vector Information. Clontech Laboratories, Inc. www.clontech.com Protocol No. PT4051-5 2 Version No. PR073571. Use. pLVX-shRNA1 is available in the Lenti-X™ shRNA Expression System (Cat. No. 632177). Before it can be transduced into target cells, the vector must be cotransfected into 293T cells with our Lenti-X HTX Packaging shipping household to hawaii

pLVX-shRNA2-Puro - lifescience-market.com

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Plvx-shrna2

Lentiviral vectors with fluorescent proteins - Takara Bio

WebpLVX-shRNA2-Puro Description Plasmid type:RNAi vectorPromoter:U6Size:8998 bpReporter gene:ZsGreen1Resistance (s):Ampicillin (Amp)Selectable markers:Puromycin Caution: 1. … WebpLVX-Puro Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

Plvx-shrna2

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WebOct 11, 2024 · The original CMV IE promoter of pLVX-shRNA2 was replaced with a mouse PGK promoter (PCR and cloned by primers 5’–ACACGAATTCTACCGGGTAGGGGAGGCGCT and 5’–ACACCCCGGGCGAAAGGCCCGGAGATGAGG) to generate the pLVX2-shRNA2-PGK vector which drives persistent ZsGreen (enhanced GFP) expression. Oligos carrying short … WebJan 20, 2024 · 方法：根据GenBank提供的3β-HSD基因mRNA序列，设计合成3条干扰序列，退火后连接到PLVX-shRNA2-puro中，将重组慢病毒载体转染293FT细胞，收集病毒上 …

WebPlvx Shrna2 Puro Vector, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebAfter annealing in vitro, insert pLVX-shRNA-puro construct recombinant plasmid, then enzyme digestion and sequencing analysis. The lentiviral-shRNAs were transient-transfected into K1 cells. TFF3 mRNA and protein levels were test by real-time PCR and western blot respectively in K1 cells at 48h post transient-transfected.

WebOct 1, 2024 · After lentiviral expression vector pLVX-shRNA2-SENP7 carrying the siRNA sequence (440) was constructed, it was transfected into 293 T cells and packed into … WebFast accurate construct design for all major molecular cloning techniques. Validate sequenced constructs using powerful alignment tools. Customize plasmid maps with …

WebSep 22, 2024 · IMPORTANT NOTE: NIH does not independently verify information submitted to the GTR; it relies on submitters to provide information that is accurate and not …

WebPlvx Shrna2 Zsgreen Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … quelle age a ed sheeranWebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into ... quelle goldwing choisirWeb与未感染任何病毒的Huh7、Hep3B细胞，感染pLVX-shRNA2空载体的Huh7、Hep38细胞相比，感染携带IGF1R干扰序列慢病毒的Huh7、Hep3B细胞IGF1R mRNA表达水平显著下调，细胞增殖活性明显降低，细胞迁移和侵袭能力明显受到抑制(P均0.01)。结论筛选出1对高效沉默IGF1R基因的siRNA ... quel chromebook choisir 2022Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 shipping house plantsWebTakara Bio—Home shipping hoverboard fedexWebAug 13, 2024 · Argonaute2 (Ago2) is a key component of the RISC that can directly degrade mRNA by a slicing mechanism 21, 22, we performed RIP assays using the AGO2 antibody to further confirm the interaction... shipping houston texasWebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ control pLVX … quellen apotheke bad soden