WebMYOB All components mixed with 1L water and then autoclaved: 2.0g NaCl, 0.55g TrisHCl, 0.24g TrisOH, 4.6g Bactotryptone, 8mg Cholesterol, 20g Agar. For convenience, make a … WebThe goal of this protocol is to enable seamless CRISPR/Cas9 editing of the C. elegans genome using assembled ribonucleoprotein complexes and the dpy-10 co-CRISPR marker for screening. This protocol can be used to make a variety of genetic modifications in C. elegans including insertions, deletions, gene replacements and codon substitutions.
High-throughput screening of small molecules for bioactivity and …
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Web41. Modified Youngren’s, Only Bacto- peptone (MYOB) plates for worm maintenance (see Recipes) 42. Cholesterol stock solution (see Recipes) 43. E. coli OP50 stock (see Recipes) 44. Cellulose capillary attachment (see Recipes) 45. 20% BSA solution (see Recipes) 46. 25 mM Levamisole solution (see Recipes) 47. WebSep 12, 2024 · 101 Strains were maintained on MYOB plates seeded with E. coli OP50 and grown at 19° unless otherwise 102 indicated. The fzr-1::gfp::3xflag construct containing 21.6Kbp of the fzr-1 5’UTR and 6Kbp of the fzr-1 ... 111 plates and allowed to self-fertilize for 24 hours at the temperatures indicated. For brood size assays, sterling bank clayton missouri