Flex-chr2
Web首先，通过在Trh-ires-Cre; Npy-hrGFP小鼠的PVH和DMH分别注射Cre依赖的ChR2病毒，在光刺激下同步记录AgRP的光刺激引起的突触后电流（light-evoked quantal EPSCs, le-qEPSCs），发现光刺激可以同步引起突触后电流，若用Sr2+离子代替Ca2+，则该突触后电流被扰乱，说明PVHTRH-AgRP以及DMHVglut2-AgRP有兴奋性突触连接（图1A-B）。 WebSep 18, 2013 · Injection of rAAV containing Cre-dependent expression constructs (e.g., 2/1.FLEX.GCaMP3; 2/1.FLEX.ChR2-tdTomato) into the OB leads to expression that appears restricted to MTCs, with the majority of somata in the mitral cell layer and deep glomerular layer (Fig. 1A) and fewer somata in the external plexiform layer.
Flex-chr2
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WebFeb 4, 2024 · We injected cre-dependent rAAV2-EF1α-FLEX-ChR2-eYFP virus into the NTS region of mice expressing cre-recombinase in TH + neurons and placed an optical fiber over the ARC (Figure 2 A). Surprisingly, photostimulation of NTS TH →ARC axons in ad libitum -fed mice during the day induced a robust increase in food consumption ( Figures 2 B and … WebPlasmid AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA from Dr. Hongkui Zeng's lab contains the insert ChR2(H134R)-EYFP and is published in Neuron. 2015 Mar 4;85(5):942-58. doi: …
WebMay 14, 2024 · We injected FoxP2Cre mice, that express Cre-recombinase enzyme in all the FoxP2 cells, bilaterally in the PB with adeno-associated virus containing the gene for either channel rhodopsin2 (AAV-FLEX-ChR2-mCherry; n=3, for neuronal activation) or archaerhodopsin T TP009 (AAV-FLEX-ArchT-GFP ArchT; n=2, for neuronal inhibition) in … WebNov 6, 2024 · To test this, we injected AAV with Cre-dependent expression of channelrhodopsin-2 (E123T/T159C) fused with enhanced yellow fluorescent protein (AAV-CMV-flex-ChR2-EYFP) or hrGFP (AAV-CMV …
WebDec 12, 2024 · We injected cre-dependent rAAV2-EF1α-FLEX-ChR2-eYFP virus into the NTS region of mice expressing cre-recombinase in TH + neurons and placed an optical fiber over the ARC (Figure 2A). Surprisingly, photostimulation of NTS TH →ARC axons in ad libitum -fed mice during the day induced a robust increase in food consumption ( Figures … WebMar 6, 2024 · (A) Diagram demonstrating LH injection with AAV-Flex-ChR2-EYFP and implantation of optic fiber over the DBB. (B) Representative coronal slices of LH Vgat …
WebNov 8, 2016 · In ChR2− (control) mice (n = 5), virus lacking the FLEX-ChR2 gene (AAV5-EF1a-DIO-EYFP) was targeted to the VTA using the same stereotaxic coordinates. In all mice, optical fibers (200 µm inner diameter, N.A. 0.22; Doric Lenses) were lowered into the brain 4.1 mm so that the tip of the fiber was near the dorsal aspect of the VTA. …
WebFeb 19, 2024 · The AAV-flex-ChR2(H134R)-EYFP transgene was a gift from Karl Deisseroth (Addgene plasmid 20298). This expresses the humanized ChR2 gene with histidine 134 changed to arginine, to make larger currents; EYFP is fused to the C terminus of ChR2, which also makes it a good substrate for axonal transport. spongebob vacation resortWebWith a gentle flex and twist your print is released from the flex-plate without the need for scrapers. In stock. LCHR2 Flex-Plate quantity. Add to basket. SKU: PC000391 … spongebob vacation songWebSep 9, 2015 · The headstage is using a novel foldable rigid-flex printed circuit board design, which results into a lightweight and compact device. Recording experiments performed in the cerebral cortex of transgenic ChR2 mice under anesthetized conditions show that the proposed headstage can trigger neuronal activity using optical stimulation, while ... spongebob vacation houseWeb比如上面的ALK基因，先截取chr2上的29415640-30144452区间，再反向互补即得到ALK基因的碱基序列。 验证过程：方法一：bedtools工具包可以根据bed文件提取区间序列，这里以上面的ALK基因为例试一下：1.首先创建一个bed文件命名为AKL.bed，其中文件第6列可以指 shelli stretch dressWeb(C) Cells were infected with AAV-FLEX-rev-ChR2(H134R)-mCherry (AAV9-18916) in the presence and absence of Cre, and mCherry expression was assessed by direct fluorescence. (D) Comparison of the rates of Cre-independent recombination between plasmid DNA and its associated viral DNA extracts. spongebob using the bathroomWebIn addition to the viral particles, you will also receive purified AAV-FLEX-rev-ChR2 (H134R)-mCherry plasmid DNA. CAG-driven, cre-dependent, humanized channelrhodopsin H134R mutant fused to mCherry for … spongebob vector png deviantartWebFeb 4, 2015 · In control experiments, we confirmed the photoexcitability of the ChR2-expressing CT neurons after injection of AAV-flex-ChR2 into M1 (Fig. 4A–C). Photostimuli excited these transfected CT neurons very … shell istasyon